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KMID : 0380420100340030237
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Journal of Prventive Veterinary Medicine
2010 Volume.34 No. 3 p.237 ~ p.243
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Survey on aflatoxins in pig feeds in Korean pig farms
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Song Yun-Kyung
Jung Byeong-Yeal
Choi Jung-Soo
Moon Oun-Kyoung
Lee Soo-Hyung
CHung Duek-Hwa
Park Son-Il
Park Choi-Kyu
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Abstract
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Aflatoxins produced by Aspergillus spp. are recognized as a major concern in animal and human health. In pigs, ingestion of aflatoxin-contaminated feeds causes immunosuppression, hepatotoxicosis and poor feed efficiency. In this study, we screened the contaminated level of aflatoxins in 449 pig feeds from 12 swine farms in Korea. For rapid and efficient screening of aflatoxins in pig feeds we evaluated the feasibility of three commercial ELISA kits for the screening of aflatoxins in pig feeds. Twenty-nine pig feed samples were examined for total aflatoxins using three ELISA kits, simultaneously. From three repetitions of each assay, the average intra-assay precisions and the average inter-assay precisions expressed as coefficient of variation (CV, %) for Veratoxⓡ Quantitative Aflatoxin test were 6.90 and 12.29,
respectively. A statistical comparison of the results between HPLC and ELISAs showed that the correlation coefficient
values for Veratoxⓡ was 0.96. The results demonstrated that we can apply the Veratoxⓡ Quantitative Aflatoxin test for
the detection of aflatoxins in pig feed from the field. The screening of field samples with this ELISA kit showed that
11 out of 265 pig feeds for growers and weaners were contaminated with total aflatoxin levels exceeding 10 ppb, maximum tolerable limits for their compound feeds and the aflatoxins levels of remaining 184 pig feeds for other age groups of pigs were confirmed as below 20 ppb. The results from the screening indicated overall low levels of aflatoxins contamination in pig feeds.
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KEYWORD
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Aflatoxin, Contamination, ELISA, Pig Feeds, Swine Farms
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